Beta葡聚糖酶[麦芽和微生物]检测试剂盒

Beta葡聚糖酶[麦芽和微生物]检测试剂盒  β-Glucanase Assay Kit (Malt and Microbial)

1) 英文:Beta-Glucanase (Malt & Microbial) Assay Kit;

2) 中文:Beta葡聚糖酶[麦芽和微生物]检测试剂盒;

3) 规格: 100; 4) 货号:K-MBGL

商品说明:
β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.
Colourimetric method for the determination of β-Glucanase
in malt, foodstuffs and fermentation products

Principle:
(β-glucanase)
(1) Azo-Barley β-glucan (polymer) → Azo-barley β-glucan
(fragments)

(2) Add alcohol; centrifuge to remove polymeric Azo-barley
β-glucan

(3) Measure the absorbance of the supernatant solution
Kit size: 100 assays
Method: Spectrophotometric at 590 nm
Reaction time: ~ 30 min
Detection limit: 100 U/kg of malt
Application examples:
Malt extracts, wort, beer and other materials
Method recognition:
RACI (Standard Method)
Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only kit available
  • Very specific
  • Simple format
  • Standard included

甘油检测试剂盒 Glycerol GK Assay Kit

甘油检测试剂盒 Glycerol GK Assay Kit
产品货号:K-GCROL
产品品名: 甘油检测试剂盒
英文品名 :Glycerol Assay Kit
规格型号: 70 assays per kit
甘油是食品加工业中通常使用的甜味剂和保湿剂,大多出现在运动食品和代乳品中。
上海甄准生物提供 甘油检测试剂盒 ,紫外吸光度法( 340nm )测定食品、饮料及其他原料中的甘油。
原理:
反应时间: 5min
检测限: 0.37mg/L
适用样品: 葡萄酒(葡萄汁)、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸(硬纸板)、化妆品
优点:
l    新型的片剂形式增加了稳定性
l    价格低廉(每次检测成本)
l    所有试剂配制后的稳定性 >2 年
l    反应快
l    该方法已通过 OIV , MEBAK 以及德国和瑞士的认证

商品说明:
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(ADP-GK)
(2) ADP + D-glucose → G-6-P + AMP
(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NAD+ → gluconate-6-phosphate + NADH + H+
Kit size: 70 assays (manual) / 700 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 7 min
Detection limit: 0.37 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method
Advantages

  • Novel tablet format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Positive reaction (assay proceeds with an increase in absorbance)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

D-果糖/D-葡萄糖检测试剂盒 D-Fructose/D-Glucose Assay Kit

D-果糖/D-葡萄糖检测试剂盒 D-Fructose/D-Glucose Assay Kit
1) 英文:D-Fructose/D-Glucose Assay Kit;
2) 中文:D-果糖/D-葡萄糖检测试剂盒;
3) 规格: 110; 4) 货号:K-FRUGL

方法认证 :AOAC,EN。NEN。NF。DIN,GOST,OIV,IFU ,AIJN,MEBAK,IOCCC以及德国、瑞士、奥地利和意大利的认证

优点:加入PVP防止丹宁酸的抑制;适用于手工和自动分析仪检测(优良的机器稳定性);已通过法国叙兹拉鲁塞葡萄酒大学的验证;价格低廉(每次检测成本);所有试剂配制后的稳定性〉2年(适用于手工分析);反应快25℃或37℃;包含稳定的D-葡萄糖/D-果糖标准溶液

检测原理:

检测方法:紫外风光光度法

检测波长:340nm;

反应时间:13min;

检测限:066mg/L;

适用样品:葡萄酒、啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、食疗食品、烤食品、糖果、甜点、糖食、冰淇淋、水果和蔬菜、调味品、烟、化妆品、医药品、纸及其他原料(如生物培养基等样品);

商品说明:
D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

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麦芽糖/蔗糖/D-葡萄糖检测试剂盒
乳糖/D-半乳糖[快速]检测试剂盒
乳糖/蔗糖/D-葡萄糖检测试剂盒
天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒
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D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 D-mannose/D-fructose/D-glucose assay kit

产品名称:D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

英文名称:D-mannose/D-fructose/D-glucose assay kit

货号: K-MANGL

型号规格:55次

优点:成本低(每次检测成本);所有试剂配制后的稳定性〉2年;只有酶试剂盒可用;方法简单;反应快;包含标准品

检测原理:

检测方法:紫外分光光度法

检测波长:340nm;

反应时间:25min;

检测限:0.66mg/L;

适用样品:食品、酵母细胞制剂、酶水解产物和其他原料(如:生物培养、样品等);

方法认证:新方法

商品说明:
The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.
UV-method for the determination of D-Mannose, D-Fructose
and D-Glucosein foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphomannose isomerase) (phosphoglucose isomerase)
(3) M-6-P ↔ F-6-P ↔ G-6-P

Kit size: 55 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Beta葡聚糖酶[麦芽和微生物]检测试剂盒

Beta葡聚糖酶[麦芽和微生物]检测试剂盒

英文名:β-Glucanase Assay Kit (Malt and Microbial)

货号:K-MBGL

规格:100 assays per kit

     β-葡聚糖酶作用方式不同,可分为内切型和外切型。前者存在于谷物种子、某些真菌和某些细菌中,能催化水解谷物细胞壁中的β-葡聚糖,其中包括内切型β-1,4-葡聚糖酶、内切型β-1,3-葡聚糖酶。后者存在于谷物种子中,其中又包括外切型β-1,4-葡聚糖酶、外切型β-1,3-葡聚糖酶。
在工业上采用细菌(如枯草芽孢杆菌)、曲霉(如黑曲霉)、青霉(如伊氏青霉)生产。在啤酒生产原料大麦及其麦芽中,含本品较多。在啤酒生产中采用β-葡聚糖酶,可提高麦汁的过滤速度和得率,从而保证啤酒质量。也可用于饴糖、麦芽糖浆生产和用作饲料添加剂。

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.
Colourimetric method for the determination of β-Glucanase
in malt, foodstuffs and fermentation products
Principle:
(β-glucanase)
(1) Azo-Barley β-glucan (polymer) → Azo-barley β-glucan
(fragments)

(2) Add alcohol; centrifuge to remove polymeric Azo-barley
β-glucan

(3) Measure the absorbance of the supernatant solution
Kit size: 100 assays
Method: Spectrophotometric at 590 nm
Reaction time: ~ 30 min
Detection limit: 100 U/kg of malt
Application examples:
Malt extracts, wort, beer and other materials
Method recognition:
RACI (Standard Method)
Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only kit available
  • Very specific
  • Simple format
  • Standard included

蔗糖/D-果糖/D-葡萄糖检测试剂盒

蔗糖/D-果糖/D-葡萄糖检测试剂盒 Sucrose/Fructose/D-Glucose Assay Kit

产品名称:蔗糖/D-果糖/D-葡萄糖检测试剂盒

英文名称:Sucrose,D-fructose and D-glucose sucrose assay kit

货号:K-SUFRG

型号规格:150 (50 of each)

优点:价格低廉(每次检测成本);

所有试剂配制后的稳定性〉12个月;

反应快;葡萄糖氧化酶/过氧化酶/己糖激酶/6-磷酸葡萄糖脱氢酶;

包含标准品

检测原理:


检测方法:紫外分光光度法

检测波长:340nm;

反应时间:30min;

检测限:1.38mg/L;

适用样品:啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、面包、焙烤产品、乳制品、糖果、甜点、糖食、冰淇淋、水果和蔬菜(如:马铃薯)、肉制品(如香肠)、调味品、饲料、烟、化妆品、纸及其他原料(如生物培养、样品等);

方法认证:EN。NEN。NF。DIN,GOST,IFU ,AIJN,MEI,IOCCC以及德国、奥地利认证

商品说明:
The Sucrose/Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials

Principle:
(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose
(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(5) F-6-P → G-6-P
Kit size: 100 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 1.38 mg/L
Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread,
bakery products, dairy products, candies, desserts, confectionery, sweets,
ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage),
condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics,
pharmaceuticals, paper and other materials
Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN,
DIN, GOST, IFU, AIJN, MEBAK and IOCCC
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Stabilised D-glucose / D-fructose standard solution included
  • Extended cofactors stability

内切纤维素酶检测试剂盒

内切纤维素酶检测试剂盒

英文名:Cellulase Assay Kit (CELLG3 Method)
货号:K-CELLG3
规格:180 / 360 assays per kit / 720 (auto-analyser)
市场价: 5537

 纤维素酶是一种重要的酶产品,是一种复合酶,主要由外切β-葡聚糖酶、内切β-葡聚糖酶和β-葡萄糖苷酶等组成,还有很高活力的木聚糖酶。
提供内切纤维素酶检测试剂盒,色度法(400 nm)测定酶制品和发酵产品中的纤维素酶(内切-1,4-β-葡聚糖酶)。
内切纤维素酶检测试剂盒(CELLG3方法) K-CELLG3
使用高纯度β-葡萄糖苷酶和苯亚甲基阻断,2 – 氯-4 – 硝基苯基-β-Dcellotrioside(BClPNPβ-G3)。 β-葡萄糖苷酶的能力确保测定的可靠的最大的灵敏度。BClPNPβ-G3的水解为苯亚甲基,通过纤维素酶阻断纤维二糖和2-氯-4 -硝基苯基-β-D-葡萄糖, 2-Cl-4-硝基苯基-β-D-葡萄糖通过β-葡萄糖苷酶立即裂解为D-葡萄糖和游离的2 – 氯-4 – 硝基苯酚(ClPNP)。
反应时间:16min
适用样品:酶制品和发酵产品
优点:
价格低廉(每次检测成本)
特异性高
方法简单
包含标准品
 
The CELLG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;

1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase.  The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase.  The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CELLG5) is now available for the measurement of endo-cellulase.  The CELLG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases.  In addition, the exchange of the benzylidene blocking group in CELLG3 for 3-keto-butylidene in CELLG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay.  As DMSO is known to inhibit certain cellulases, this is another benefit in using CELLG5.  Megazyme now recommends the use of K-CELLG5 for all assays for the measurement of endo-cellulase.
Colourimetric method for the determination of 
endo
-1,4-β-glucanase (cellulase) in enzyme preparations and fermentationproducts
Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G5-β-PNP + H2O → Blocked-GX + G(5-X)-β-PNP
(thermostable β-glucosidase)
(2) G(5-X)-β-PNP + H2O → D-glucose + PNP
(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)
Method:                         Spectrophotometric at 400 nm
Total assay time:           10 min
Detection limit:                3.5 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition:     Novel method

 

D-乳酸检测试剂盒,D-Lactic Acid Assay Kit

D-乳酸检测试剂盒,D-Lactic Acid Assay Kit
名称:D-乳酸检测试剂盒
英文名:D-Lactic Acid Assay Kit
规格:50次
产地:爱尔兰
外观 :五玻璃瓶。
比重 :不适用
在水中的溶解度: 易溶
pH值: 7.0
气味: 无
粉末和液体形式
稳定性: 稳定 5年或以上(冷冻)
成分:
名称 比例
甘氨酰甘氨酸缓冲液 1瓶(玻璃)
烟酰胺腺嘌呤二核苷酸 1瓶(玻璃)
D-谷氨酸丙酮酸转氨酶 1瓶(玻璃)
D-乳酸脱氢酶酶 1瓶(玻璃)
D-乳酸的标准品 1瓶(玻璃)
许多食品和饮料中都发现有D-乳酸.  由乳酸菌产生, D-乳酸存在于牛奶的发酵产品中比如酸奶,腌菜,腌肉和腌鱼中。牛奶、肉、和果汁的质量可以通过测定D-乳酸的含量来确定。在葡萄酒生产中,D-乳酸的产生标志着酒被乳酸菌***。在化学工业中,D-乳酸和L-乳酸都是生产过程中聚合物的原料,比如聚交酯和一些生物学上可降解的聚合物。同时也应用在化妆品和医药品中。
原理:
D-乳酸的定量分析需要两步酶化反应。  第一个反应由D-乳酸脱氢酶(D-LDH)催化,D-乳酸在烟酰腺嘌呤二核苷酸(NAD+)的催化下,被氧化成丙***酸
然而, 因为反应(1)的移动和D-乳酸和NAD+有着密切的关系,下一步反应需要消耗掉产生的丙***酸 在大量D-谷氨酸的存在下,通过在D-谷氨酸-丙***酸转氨酶(D-GPT)的作用下把丙***酸转化为D-丙氨酸和2-***戊二酸。(2).
以上两个反应中产生的 NADH 的量可以用来计量D-乳酸的量。  NADH的量通过340nm处增加的吸光率来测量。
特异性,敏感度, 线性和准确性:
此方法特异性检测D-乳酸.
此方法特异性检测D-乳酸和L-乳酸。在D-乳酸锂(MW=96.0)的检测中,大约可以预期结果为96%(W/W),L-乳酸锂可以得到98%(W/W)。
检测的灵敏度为0.005 个吸光度单位。相当于样品最大体积为1.50 mL 时,样品浓度为0.107 mg/L(或者 1.60 mg/L ,样品体积0.1 mL) 。检测限为 0.214 mg/L,这是在0.010的吸光度差异,样品最大体积为1.50 mL为得到的 。
D-乳酸检测的线性变化为0.5 到 30 μg 。在重复测定中,同一份样品溶液,可能产生0.005 到 0.010 个吸光光度值的差异。这就相当于样品1.50 mL,样品溶液中D-乳酸的浓度为0.107 到 0.214 mg/L 。如果样品在制备阶段被稀释过,结果要乘以稀释倍数F。如果样品制备阶段,称量过样品,比如10 g/L,可能会产生0.02 到 0.05 g/100 g 的差异。
干扰:
如果D-乳酸的转换在实验指定的时间内(大约5分钟)彻底完成,通常认为是没有发生任何干扰。不过,这个也可以进一步验证,通过在实验的完成时,向比色皿中加入D-乳酸混合物(大约每0.1mL15ug),吸光光度值有会明显增加。
样品中的干扰物质的分析可以通过加入一个内参来确定。需要进行一个标准品定量回收实验,通过这个回收实验(也就是在最初的提取步骤中向样品中加入D-乳酸)可以算出样品在处理和提取过程中的损失量。
D-Lactic Acid (D-Lactate) (Rapid)
For the rapid and specific assay of D-lactic acid in wine, beer, juice, milk, cheese, vinegar and meat products.
Catalogue Number: K-DATE
Content: 50 assays per kit
Appearance Five glass vials.
Specific Gravity not applicable
Solubility in Water Readily soluble.
pH Value 7.0
Odour none
Form powders and liquids
Stability stable in a freezer for five or more years
Ingredients Name CAS Proportion
Glycylglycine buffer 1 vial (glass)
Nicotinamide adenine dinucleotide 1 vial (glass)
D-Glutamate pyruvate transaminase enzyme 1 vial (glass)
D-Lactate Dehydrogenase enzyme 1 vial (glass)
D-Lactic acid standard solution 1 vial (glass)
K-DATE  D-乳酸快速检测试剂盒
乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
K-DATE  D-乳酸快速检测试剂盒
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31
L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
K-DATE  D-乳酸快速检测试剂盒
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
K-DATE  D-乳酸快速检测试剂盒
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49

Megazyme官网及其产品分类

特色

Megazyme官网及其产品分类

在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Megazyme的签约代理商,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Megazyme高品质的产品和服务,欢迎广大新老客户来电咨询。
Megazyme代理,Megazyme上海代理,Megazyme北京代理,Megazyme广州代理,Megazyme总代理,Megazyme一级代理,Megazyme特约代理,Megazyme授权代理 Megazyme现货上海金畔生物科技有限公司是Megazyme专业代理商。

       爱尔兰Megazyme是一家全球领先的制造和供应高品质和创新的检测谷物,食品,饲料,发酵,乳制品和葡萄酒行业的技术。Megazyme成立于1988年,该公司目前提供了超过70种诊断试剂盒和超过300种其他试剂和底物。

该公司产品分以下系列:

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)
2. 试剂混合物(Reagent Mixtures)
3. 协同因子(Cofactors)
4. 糖酶片剂试验(Carbohydrase Tablet Tests)
5. 蛋白酶片剂试验(Protease Test Tablets)
6. 可溶性呈色底物(Soluble Chromogenic Substrates)
7. 不可溶性(交联的)呈色底物(Insoluble (Crosslinked) Chromogenic Substrates)
8. 酶(Enzymes)
9. 植物凝血素(Lectins)
10. 多聚糖(Polysaccharides)
11. 低聚糖(Oligosaccharides)
12. 相关产品 (General)

Megazyme公司简介:
Megazyme公司位于美丽的爱尔兰 ,是全球知名的酶及酶法分析试剂盒供应商, 他们的很多产品都被AOAC(Association of Official Analytical Chemists)、(European Brewing Convention)、RACI (Royal Australian Chemical Institute) 等国际组织采用,Megazyme公司分析试剂盒广泛应用于食品、饲料、乳品、发酵工业、葡萄糖生产及成品控制,其中欧洲检测果汁及葡萄酒等发酵产品基本都采用Megazyme公司的产品。

Megazyme酶法分析检测原理

NADH((或者 NADPH)在340nm波长处具有强吸收峰,Megazyme酶法分析检测产品利用这一原理,选择消耗或者产生NADH((或者 NADPH)的化学反应,利用分光光度计检测NADH((或者NADPH)浓度的变化,从而达到检测待检物质浓度的目的。
Megazyme酶法分析检测系列包括: 

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)

麦芽淀粉酶检测试剂盒 K-MALTA 
酸类检测试剂盒
醇类检测试剂盒
醛类检测试剂盒
氨类检测试剂盒
氮类检测试剂盒
盐/酯类检测试剂盒
酶类检测试剂盒
糖类检测试剂盒
淀粉类检测试剂盒


 其中产品被国际机构采用,建立国际检测方法如下:


 总淀粉检测盒(Total Starch Assay Kit)
• AOAC Method 996.11
• AACC Method 76-13
• ICC Standard Method no. 168
• RACI Standard Method
总膳食纤维检测盒(Total Dietary Fibre Assay Kit)
• AOAC Method 985.29
• AOAC Method 991.42
• AOAC Method 993.19
• AOAC Method 991.43
• AACC Method 32.07
• AACC Method 32.21
• AACC Method 32.05
淀粉损失检测盒(Starch Damage Assay Kit)
• AACC Method 76-31
• ICC Standard No. 164
• RACI Standard Method
α-淀粉酶检测盒Ceralpha (α-Amylase) Assay Kit
•    AOAC Method 2002.01
•    ICC Standard No. 303
•    RACI Standard Method
•    CCFRA Flour Testing Working Group Method 0018
•    AACC – under evaluation.
Alpha淀粉酶检测盒Amylazyme (α-Amylase Assay)
• AACC Method 22.05
• RACI Standard Method
果聚糖检测盒Fructan Assay Kit
• AOAC Method 999.03
• AACC Method 32-32
木聚糖酶AX片剂(Xylazyme AX Tablets)
• Xylazyme AX Tablets have been widely adopted in the fermentation and feeds industries for the measurement of Xylanase.
β-葡聚糖检测盒 β-Glucan (Mixed Linkage) Assay Kit
• AACC Method 32-23
• EBC Methods 3.11.1, 4.16.1 and 8.11.1
• AOAC Method 995.16
• ICC Standard No. 166
• RACI Standard Method
极限糊精酶和支链淀粉酶检测片剂(Limit-Dextrizyme)
• RACI Standard Method
β-葡聚糖酶检测盒(β-Glucanase (Malt and Microbial) Assay Kit)
• RACI Standard Method
β-淀粉酶检测盒(Betamyl (β-Amylase Assay Kit))
• RACI Standard Method.
木聚糖酶检测底物(Azo-Wheat Arabinoxylan )
• Azo-Wheat Arabinoxylan, Azo-CM-Cellulose and Ceralpha methods for the assay of Xylanase, cellulase and ?-Amylase, respectively, have been adopted by the UK silage industry.
抗性淀粉检测盒(Resistant Starch Assay Kit)
• AOAC Method 2002.02

温馨提示:不可用于临床治疗。

2.试剂混合物
3.蛋白酶检测片剂
4.糖酶片剂
5.可溶性呈色底物
6.植物凝血素
7.纤维类检测试剂盒
8.胶质类检测试剂盒
9.不可溶性(交联)呈色底物
10.酶制剂
11.协同因子
12.多糖/聚糖/多聚糖
Megazyme官网及其产品分类

我们常年提供以下试剂盒:
麦芽淀粉酶检测试剂盒 K-MALTA 
乙酸检测试剂盒  货号: K-ACET
乙酸[AF法]检测试剂盒  货号:K-ACETAF
乙酸【AK法】检测试剂盒  货号: K-ACETAK
乙酸GK检测试剂盒  货号:(分析仪格式) K-ACETGK
乙酸[乙酸激酶法]检测试剂盒  货号: K-ACETRM
可吸收碳水化合物/膳食纤维检测试剂盒  货号:K-ACHDF
乙醛检测试剂盒  货号:K-ACHYD
α-葡萄糖醛酸酶检测试剂盒  货号:K-AGLUA
氨(快速)检测试剂盒  货号:K-AMIAR
直链淀粉/支链淀粉检测试剂盒  货号: K-AMYL
阿拉伯聚糖检测试剂盒  货号:K-ARAB
L-阿拉伯糖/D-半乳糖检测试剂盒  货号:K-ARGA
维生素C(L-抗坏血酸)检测试剂盒  货号:K-ASCO
天冬酰胺/谷氨酰胺和氨快速检测试剂盒  货号:K-ASNAM
阿斯巴甜检测试剂盒  货号:K-ASPTM
木聚糖酶(Azo-Wax)检测试剂盒  货号:K-AZOWAX
β-淀粉酶检测试剂盒  货号:K-BETA3
β-葡聚糖[混联]检测试剂盒  货号:K-BGLU
内切纤维素酶检测试剂盒  货号:(CELLG3方法) K-CELLG3
α-淀粉酶检测试剂盒  货号:K-CERA
柠檬酸检测试剂盒  货号:K-CITR
D-乳酸快速检测试剂盒  货号:K-DATE
D-/L-乳酸快速检测试剂盒  货号:K-DLATE
D-苹果酸检测试剂盒  货号:K-DMAL
酵母葡聚糖酶检测试剂盒  货号:K-EBHLG
乙醇检测试剂盒  货号:K-ETOH
亚硫酸盐总量(酶法)检测试剂盒  货号:K-ETSULPH
蚁酸检测试剂盒  货号: K-FORM
D-果糖/D-葡萄糖(MegaQuant格式)检测试剂盒  货号:K-FRGLMQ
D-果糖/D-葡萄糖检测试剂盒  货号:[液体即用型]  K-FRGLQR
果聚糖(HK法)测试剂盒  货号: K-FRUCHK
D-果糖/D-葡萄糖检测试剂盒  货号: K-FRUGL
L-岩藻糖检测试剂盒  货号:K-FUCOSE
半乳甘露聚糖检测试剂盒  货号:K-GALM
D-葡萄糖胺检测试剂盒  货号: K-GAMINE
D-葡萄糖酸 / D-葡萄糖酸内脂检测试剂盒  货号:K-GATE
甘油检测试剂盒  货号: K-GCROL
甘油GK法检测试剂盒  货号: K-GCROLGK
L-谷氨酸酰胺/氨(快速)检测试剂盒  货号: K-GLNAM
葡萄糖氧化酶检测试剂盒  货号: K-GLOX
D-葡萄糖(GOPOD格式)检测试剂盒  货号: K-GLUC
D-葡萄糖[HK法]检测试剂盒  货号: K-GLUHKR/L
葡甘露聚糖检测试剂盒  货号:K-GLUM
L-谷氨酸检测试剂盒  货号:K-GLUT
D-3羟丁酸检测试剂盒  货号:K-HDBA
肌醇检测试剂盒  货号: K-INOSL
D-异柠檬酸检测试剂盒  货号:K-ISOC
乳糖/D-半乳糖检测试剂盒  货号:K-LACGAR
乳糖/蔗糖/D-葡萄糖检测试剂盒  货号:K-LACSU
乳果糖检测试剂盒  货号:K-LACTUL
L-精氨酸/尿素/氨[快速]检测试剂盒  货号:K-LARGE
L-乳酸检测试剂盒  货号:K-LATE
L-苹果酸(分析仪格式)检测试剂盒  货号:K-LMALAF
L-苹果酸检测试剂盒  货号: K-LMALL
L-苹果酸(MegaQuant)检测试剂盒  货号:K-LMALMQ
L-苹果酸检测试剂盒  货号:(液体即用型)K-LMALQR
L-苹果酸检测试剂盒  货号:K-LMALR
麦芽淀粉酶检测试剂盒  货号: K-MALTA
D-甘露糖/D-果糖/D-葡萄糖检测试剂盒  货号:K-MANGL
D-甘露醇/L-阿拉伯糖醇检测试剂盒  货号:K-MANOL
麦芽糖/蔗糖/D-葡萄糖检测试剂盒  货号:K-MASUG
β-葡聚糖酶(麦芽和微生物)检测试剂盒  货号:K-MBGL
初级氨基氮(NOPA)检测试剂盒  货号:K-PANOPA
果胶鉴定试剂盒  货号:K-PECID
植酸检测试剂盒  货号: K-PHYT
丙酮酸检测试剂盒  货号:K-PYRUV
棉子糖/D-半乳糖检测试剂盒  货号:K-RAFGA
蜜三糖/棉子糖/蔗糖/D-半乳糖检测试剂盒  货号:K-RAFGL
L-鼠李糖检测试剂盒  货号: K-RHAMNOSE
抗性淀粉检测试剂盒  货号:K-RSTAR
抗性淀粉控粉套装K- RSTCL
破损淀粉检测试剂盒  货号:K-SDAM
山梨醇/木糖醇检测试剂盒  货号:K-SORB
琥珀酸/丁二酸检测试剂盒  货号:K-SUCC
蔗糖/D-葡萄糖检测试剂盒  货号:K-SUCGL
蔗糖/D-果糖/D-葡萄糖检测试剂盒  货号:K-SUFRG
游离亚硫酸盐总量检测试剂盒  货号:K-SULPH
酒石酸检测试剂盒  货号:K-TART
膳食纤维总量质控粉套装K-TDFC
总膳食纤维检测试剂盒  货号:  K-TDFR
海藻糖检测试剂盒  货号: K-TREH
淀粉总量质控粉套装K-TSCK
淀粉总量HK检测试剂盒  货号: K-TSHK
淀粉总量(AA/AMG)检测试剂盒  货号:K-TSTA
亚硫酸盐总量检测试剂盒  货号:K-TSULPH
尿素/氨[快速]检测试剂盒  货号:K-URAMR
D-葡萄糖醛酸和D-半乳糖醛酸检测试剂盒  货号:K-URONIC
D-木糖检测试剂盒  货号:K-XYLOSE
木聚糖酶检测试剂盒  货号:K-XYLS
β-葡聚糖检测试剂盒  货号:(酵母和蘑菇) K-YBGL

L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒

L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒

1) 英文:L-Glutamic Acid (L-Glutamate/MSG) Assay Kit;

2) 中文:L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒;

3) 规格: 60;

4) 货号:K-GLUT

商品说明:
The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Suitable for manual, auto-analyser and microplate formats.
Colourimetric method for the determination of L-Glutamic Acid
(Monosodium Glutamate; MSG) in foodstuffs and other materials

Principle:
(beef liver glutamate dehydrogenase)
(1) L-Glutamic acid + NAD+ + H2O ↔ 2-oxoglutarate + NADH + NH4+
(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 60 assays (manual) / 600 (microplate)
/ 700 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time: ~ 9 min
Detection limit: 0.21 mg/L
Application examples:
Fruit and vegetables (e.g. tomato), processed fruit and vegetables
(e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed
meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by ISO, GOST
and NMKL
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Glutamate dehydrogenase solution stable at -20°C
  • No wasted diaphorase solution (stable suspension supplied)
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

L-苹果酸检测试剂盒

L-苹果酸检测试剂盒L-Malic Acid Assay Kit (Manual Format)

产品货号: K-LMALL
产品品名: L- 苹果酸检测试剂盒(大)
英文品名: L-Malic Acid Assay Kit (Large)
规格型号: 116 assays
原理:
反应时间: 3min
检测限: 0.25 mg/L,
适用样品: 饮料和食品以及其他原料
优点:
l    成本低
l    使用期间所有试剂的稳定性 >2 年
l    操作简单
l    包含标准品
REFERENCES:

  1. Mollering, H. (1985). L-Malate. In  Methods of Enzymatic Analysis  (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 39-47, VCH Publishers (UK) Ltd., Cambridge, UK.
  2. AOAC Official Methods of Analysis (2002). Method 993.05 “L-Malic acid/total malic acid ratio in apple juice”. 17th ed. Chapter 37, p. 15.
  3. Gorin, N. (1976). Differences in L-malate determined enzymatically or titrimetrically in golden delicious apples.  Zeitschrift fur Lebensmittel-Unterschung und-Forschung,  162, 259-261.
  4. Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer.  J. Inst. Brew.  92, 225-228.
  5. Elkins, E. R. & Freund, W. (1994). Detection of adulteration in apple juice by L-malic/total malic acid ratio: Collaborative Study.  J. AOAC Int . 77, 411-415.
商品说明:
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format

氨[快速]检测试剂盒 Ammonia (Rapid)

氨[快速]检测试剂盒 Ammonia (Rapid)
产品品名:氨快速检测试剂盒
英文品名:Ammonia assay Kit (Rapid)

货号:K-AMIAR
规格型号:96 assays (manual) (手动)/960次(微孔板)/960次(自动分析仪)
氨对地球上的生物相当重要,它是所有食物和肥料的重要成分。氨也是所有药物直接或间接的组成。氨有很广泛的用途,同时它还具有腐蚀性等危险性质。
反应时间:3min
检测限:0.07mg/L
适用样品:葡萄酒、水果汁、软饮料、乳制品(如酸奶)、保健食品、酱、蛋以及蛋产品、奶酪、肉、加工过的肉,海鲜、焙烤食品(和发酵粉)、焙烤食品(和发酵粉)、化肥、纸、医药品、烟、化妆品、水、凯氏定氮、纸(硬纸板)和其他原料(如生物培养基,样品等)
优点:
  •   由于适用不受抑制的谷氢酸脱氢酶,所以反应速度很快
  •   适用于手工和白动分析仪检测
  •   提供的酶为稳定的悬浮液
  •   成本低(每次检测成本
  •   所有试剂配制后的稳定性〉2年
  •   片剂形式增加了稳定性
  •   包含标准品
  •   该实验方法已通过MEBAK和德国的认证
商品说明:
For the rapid assay of ammonia in all samples, including grape juice and wine. Content:96 assays per kit
UV-method for the determination of Ammonia in foodstuffs,
beverages and other materials
Principle:
(microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+
+ H2O


Kit size:    96 assays (manual) / 960 (microplate)
/ 960 (auto-analyser)
Method:  pectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit:  0.07 mg/L
Application examples:
Grape juice, wine, fruit juices, soft drinks, dairy products (e.g. milk),
dietetic food, soy sauce, eggs and egg products, cheese, meat,
processed meat, seafood, bakery products (and baking agents),
fertilisers, pharmaceuticals, tobacco, cosmetics, water, Kjeldahl
analysis, paper (and cardboard), water and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by MEBAK
Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
  • Enzyme supplied as stabilised suspension
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats
    上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、实验耗材和仪器色谱标准品。Sigma生化试剂、Abcam、CST、Santa Cruz品牌的单克隆抗体、多克隆抗体以及BD Pharmingen的流式抗体,Invitrogen品牌试剂、R&D和Biovison品牌的ELisa试剂盒、Laysan bio、Avanti、NANOCS品牌的修饰性PEG(修饰性聚乙二醇)、Lumiprobe cy系列活性荧光染料。免疫诊断试剂包括Roche、TOYOBO、NEB品牌的酶、MP bio品牌的生化试剂和Amresco分子生物学试剂。标准品包括Sigma试剂、WAKO食品、农残、环境、水质等分析检测标准品、美国APSC品牌聚合物分子质量测量高分子量标准品、德国Dr和TRC以及药典USP、EP、中检所品牌标准品、Reagecon品牌的离子和各种金属标准品;耗材和仪器包括NUSSUI、BD difco、OXOID品牌的培养基、whatman、Millipore品牌的各种滤膜、滤器和柱子填料等、Reseach diets品牌的动物饲料、Labnet、康宁Corning、Axygen、Falcon 、Eppendorf品牌的离心机离心管、移液枪及枪头等实验室常用仪器耗材。
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Email:  info@jinpanbio.com
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维生素C[L-抗坏血酸]检测试剂盒

维生素C[L-抗坏血酸]检测试剂盒
产品货号:K-ASCO
品牌:Megazyme
产品品名:维生素C(L-抗坏血酸)检测试剂盒
英文品名:Ascorbic Acid (L-Ascorbate) Assay Kit
规格型号:40 assays (manual) / 400 assays (microplate) / 400 assays (auto-analyser)

商品说明:
For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit
Colourimetric method for the determination of L-Ascorbic Acid 
in foodstuffs, feed, wine and other materials

Principle:
                        (5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H2 + MTT → dehydroascorbate +                                                                                         MTT-formazan + H+
                            (ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O2 → dehydroascorbate + H2O

Kit size:                             40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method:                             Spectrophotometric at 578 nm
Reaction time:                   ~ 8 min
Detection limit:                  0.175 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products
(e.g. cheese), dietetic foods, baby foods, processed meat, baking
additives, fruit and vegetables (e.g. tomato and potato),
pharmaceuticals, feed and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by MEBAK
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 6 months after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats 

纤维素酶检测试剂盒

纤维素酶检测试剂盒[CellG5法], Cellulase Assay Kit (CellG5 Method)

货号:K-CellG5
中文品名:纤维素酶检测试剂盒[CellG5法]
品名:Cellulase Assay Kit (CellG5 Method)
规格1:K-CellG5-4V 120/240次(手工)/480次(自动分析仪)
规格2:K-CellG5-2V 60/120次(手工)/240次(自动分析仪)
说明:CellG5检测试剂盒用于检测纤维素内切酶(1,4 -β-葡聚糖内切酶)包含两种组分:1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2)热稳定的β-葡萄糖苷酶。 酮阻断基团可阻止β-葡萄糖苷酶对BPNPG5的水解。通过纤维素内切酶的培养可生成非阻断的显色寡糖,被β-葡萄糖苷酶迅速水解。产生4-硝基苯酚的速率与β-葡萄糖苷酶水解BPNPG5直接相关。加入Tris缓冲液(pH 9.0)后可终止反应,并形成苯酚显色产物。
CellG5检测法代表了代表了纤维素酶测量方法的巨大进步。传统的纤维素酶检测依赖于酶底物,例如羧甲基纤维素,微晶纤维素,纤维低聚糖,CMC滤纸,染色多糖,包括CMC刚果红或纤维素天青。
应用:纤维素酶检测试剂盒[CellG5法]。

原理:
比色法测定酶制剂、发酵产品中1,4 -β-葡聚糖内切酶(纤维素酶)的含量。
纤维素酶检测试剂盒 (K-CellG5)检测原理
检测方法:分光光度法 @400 nm
反应时间:10min
检测限:3.5 x 10-4 U/mL
应用案例:
发酵液,工业酶制剂和生物燃料研究。
方法识别:
新方法
试剂盒组成:
Bottle 1: (x2) 或 (x4) 每瓶含有4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5)溶于3毫升含有0.02% w/v叠氮钠的10% DMSO水溶液。
-20°C下稳定超过4年。
Bottle 2: (x 2) 热稳定的β-葡萄糖苷酶(0.50 mL, 600 U/mL)溶于含有0.02% w/v叠氮钠的 50% w/v 硫酸铵溶液。
4°C下稳定超过4年。
Bottle 3: 木霉纤维素酶标准溶液 (5 mL, ~ 3 U/mL; 准确至见瓶标签) 溶于含有0.02% w/v叠氮钠的50% 的丙三醇水溶液。
-20°C下稳定超过4年。
优点:

所有试剂可稳定超过4年
价格非常具有竞争力
完全针对纤维素酶(1,4-葡聚糖内切酶)
常规操作,非常灵敏
检测方法十分简单,适合仪器分析
包含标准
参考文献:
Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase). McCleary, B. V., Mangan, D., Daly, R., Fort, S., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 385, 9-17.
Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase. Mangan, D., McCleary, B. V., Liadova, A., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 395, 47-51.
A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase). Mangan, D., Cornaggia, C., McKie, V., Kargelis. T. & V. McCleary, B. V. (2016). Analytical and Bioanalytical Chemistry, 408(15), 4159-4168.
说明书下载地址:https://secure.megazyme.com/files/Booklet/K-CellG5_DATA.pdf

L-精氨酸/尿素/氨检测试剂盒[快速]

L-精氨酸/尿素/氨检测试剂盒[快速] L-Arginine/Urea/Ammonia (Rapid)
产品货号:K-LARGE
品牌:进口
中文名:L-精氨酸/尿素/氨[快速]检测试剂盒
英文名:Integrated Total Dietary Fibre Assay Kit
规格:150 assays

商品说明:
For the specific and rapid assay of L-arginine, urea and ammonia in grape juice/must and wine. Content:150 assays – 50 of each component
UV-method for the determination of L-Arginine, Urea and Ammonia 
in grape juice, must and wine

Principle:
                            (microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+ + 
H2O
                     (urease)
(2) Urea + H2O → 2 NH3 + CO2
(arginase)
(3) L-Arginine + H2O → urea + ornithine

Kit size:                             50 assays
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 20 min [ammonia (2 min), urea (6 min),
L-arginine (7 min)] Detection limit:                  0.07 mg/L (ammonia)
0.13 mg/L (urea)
0.37 mg/L (L-arginine)
Application examples:
Grape juice, wine must, wine and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:          Improved method
Advantages

  • Improved assay format
  • Very rapid reactions due to use of uninhibited glutamate dehydrogenase
  • All enzymes supplied as stabilised suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability

索取产品说明书请联系:18301939375  QQ:3259632176