Amplite检测试剂盒适合标准的细胞生长培养基 货号:12536-AAT Bioquest荧光染料

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  • 产品名称:Amplite检测试剂盒适合标准的细胞生长培养基
  • 产品货号:12536
  • 产品品牌:AAT Bioquest

简单介绍

β-半乳糖苷酶,β-葡萄糖醛酸酶和荧光素酶是常见的报告基因,而这其中,萤火虫荧光素酶是功能*多的报告基因。*近,其它荧光素酶的使用在逐渐稳固的上升,例如海肾荧光素酶。由于海肾荧光素酶受体分子非常小,因此不需要ATP的辅助。Amplite 海肾荧光素酶报告基团检测试剂盒提供了一个快速灵敏的检测海肾荧光素酶活性的方案。该方案使用了独特的冷光基质形成以实现基于细胞的胞内海肾荧光素酶活性检测。

产品描述

产品简介:      

        β-半乳糖苷酶,β-葡萄糖醛酸酶和荧光素酶是常见的报告基因,而这其中,萤火虫荧光素酶是功能*多的报告基因。*近,其它荧光素酶的使用在逐渐稳固的上升,例如海肾荧光素酶。由于海肾荧光素酶受体分子非常小,因此不需要ATP的辅助。Amplite 海肾荧光素酶报告基团检测试剂盒提供了一个快速灵敏的检测海肾荧光素酶活性的方案。该方案使用了独特的冷光基质形成以实现基于细胞的胞内海肾荧光素酶活性检测。独特的冷光基质与海肾荧光素酶反应时,发射强烈的冷光。本试剂盒提供所有必需组分,并且适用于HTS(高通量筛选)研究。本试剂盒灵敏度高,可以便捷地用于96或384微孔板分析。本检测法适合标准的细胞生长培养基,也可用于原始或合成的hRluc基因表达检测。

英文注释:

         Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP. Our Amplite Renilla Luciferase Reporter Gene Assay Kit is designed to provide a fast and sensitive method to detect the luciferase from sea pansy (Renilla reniformis). It uses a proprietary luminogenic formulation to quantify Renilla luciferase activity in cell-based assays. Our formulation generates a luminescent product that gives strong luminescence upon interaction with Renilla luciferase. The kit provides all the essential components. It has high sensitivity and can be performed in a convenient 96-well and 384-well microtiter-plate format. The low-type signal with a half-life of one hour provides a consistent signal across large number of assay plates. The assay is compatible with standard cell growth media. This kit enables the measurement of primary expression or gene expression with wild type and the synthetic hRluc genes .