产品基本信息

货号：17029

产品名称：iFluor 440-dUTP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：994.73

溶剂：水

吸收波长(nm):430

激发波长(nm)：434

发射波长(nm)：480

消光系数(nm):40000

校正因子(280 nm)：0.229

产品介绍

染料修饰的脱氧尿苷5′-三磷酸酯（例如氨基烯丙基-dUTP）可用于通过常规酶促掺入方法（例如逆转录，缺口翻译，随机引物标记或PCR）生产含染料的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。由于DEAC和SpectrumAqua具有非常相似的光谱特性，DEAC-dUTP缀合物（＃17011）与SpectrumAqua 滤波片组（SpectrumAqua 是Vysis的商标）一起使用产生蓝色荧光色。但是，DECA荧光团的极高疏水性使DEAC缀合物易于吸附在塑料表面上，从而导致很高的测定误差。 iFluor 440染料具有出色的水溶性，可替代DEAC染料。 iFluor 440-dUTP缀合物是DEAC-dUTP缀合物的优良替代品。 iFluor 440染料缀合物比相应的DEAC生物缀合物或其他光谱相似的染料（例如SpectrumAqua）更亮。 iFluor 440荧光团的光谱与SpectrumAqua的滤波片组非常匹配（SpectrumAqua 是Vysis的商标）。

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参考文献

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