产品简介：      

         溶酶体是细胞器含有酸性水解消化酶可以分解细胞内废物和细胞碎片。溶酶体可以消化分解过剩或破旧的细胞器,细胞残渣,吞没病毒或**。溶酶体的膜提供pH值为4.5的消化酶工作环境。溶酶体的内部(pH值4.5 – -4.8)相比略微碱性细胞溶质(pH值7.2)是酸性的。溶酶体通过膜上的质子泵和氯离子通道从细胞液中获得质子保持这种pH差别。我们的细胞Navigator™荧光成像试剂盒是一组用于标记亚细胞细胞器,如膜标签、溶酶体、线粒体、细胞核等的荧光成像工具。选择性标记活细胞亚细胞器提供了一种对于研究细胞活动在时间上和空间上提供了一种十分有效的方法。

          这个特殊的试剂盒被设计用于橙色荧光（Ex/ Em = 575/597 nm）标签的活细胞的溶酶体。 LysoBrite™Red,试剂盒使用专属的趋溶酶体染料，可以有选择性的在不同PH梯度溶酶体中积累。。趋溶酶体指示剂是一种疏水性复合物，可以轻易地渗透进入完整的活细胞中，当它进入细胞后，被锁在溶酶体中；在进入溶酶体之后，它的荧光显著性增强。其显著性特征是它增强了对溶酶体的选择性。这种标记方法非常稳定，需要进行手动操作的时间很少。

         它可以用于多种研究的荧光分析中，例如微孔板分析，**组化和流式细胞术。它也可以用于多种不同类型的研究中，包括细胞粘附性、趋化性、多**抗性、细胞活性、细胞凋亡和细胞毒性的研究。本试剂盒提供所有的必需组分和*佳细胞标记方法。它适用于增殖型和非增殖型细胞，也可以用于悬浮和贴壁细胞 LysoBrite™染料明显优于相对应的 LysoTracker ™dyes (来自 Invitrogen). LysoBrite™ 染料可以存留在细胞内部不至一周并且具有非常低的细胞毒性，而相对性的  LysoTracker 染料只能存留几小时。 LysoBrite™染料是稳定的并且可以在细胞分裂后存留于下一代细胞中 。 LysoBrite™ 染料相比  LysoTracker 染料具有更优异的成像效果。

英文注释：

         Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.

        Our Cell Navigator fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label lysosomes of live cells in orange fluorescence at Ex/Em = 575/597 nm. LysoBrite Red, the proprietary lysotropic dye used in the kit, selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes.

         This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. LysoBrite dyes significantly outperform the equivalent LysoTracker yes (from Invitrogen). LysoBrite dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. LysoBrite dyes are fixable, and can survive a few generations of cell division. In addition, LysoBrite dyes are much more photostable than the LysoTracker dyes.