标签归档：替代物

TrypLE Express胰酶替代物，含EDTA，无酚红，100ml

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上海金畔生物科技代理销售TrypLE Express胰酶替代物，含EDTA，无酚红，100ml，欢迎访问官网了解更多产品信息。

TrypLE Express胰酶替代物，含EDTA，无酚红，100ml 
TRYPLE EXPRESS 
产品编号： 12604013  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 12604013

  

规格

Chelators: EDTA
Phenol Red Indicator: No Phenol Red
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Form: Liquid
pH Range: Check and Record
Osmolality: Check and Record
Concentrated: 1 X
Product Size: 100 mL
Reagent Type: Trypsin Replacement
Tests Performed: In Vitro Bioassay
Green Features: Energy efficient, Sustainable packaging, Less hazardous
Shipping Condition: Room Temperature

 

描述

 TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Life Technologies offers a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)

This TrypLE™ Express is modified as follows:

With Without
• EDTA • Phenol Red


The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Express’ exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

KnockOut 减血清替代物，干细胞培养

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上海金畔生物科技代理销售KnockOut 减血清替代物，干细胞培养，欢迎访问官网了解更多产品信息。

KnockOut 减血清替代物，干细胞培养 
KnockOut™ Serum Replacement 
产品编号： 10828028  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 10828028

 

规格

Classification: Serum-Free
Cell Type: Stem Cells (Embryonic)
Product Size: 500 mL
Green Features: Sustainable packaging
Shipping Condition: Dry Ice

 

描述

Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation optimized to grow and maintain undifferentiated ES cells in culture (1). It directly replaces FBS in existing protocols. The performance of Knockout™ SR is enhanced when used with Knockout™ D-MEM. Product performance is confirmed by evaluation of its ability to support the growth of undifferentiated D3 ES cell colonies on inactivated mouse embryonic fibroblasts.

We also offer the KnockOut™ ESC/iPSC Media Kit, an entire media system for feeder-based culture of pluripotent stem cells in one product.
For human ex vivo tissue and cell culture processing applications.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

谷氨酰胺替代物，降解无毒。100 mL

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上海金畔生物科技代理销售谷氨酰胺替代物，降解无毒。100 mL，欢迎访问官网了解更多产品信息。

谷氨酰胺替代物，降解无毒。100 mL 
GlutaMAX™ Supplement 
产品编号： 35050061  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 35050061

 

规格

Glutamine: GlutaMAX™-I
Form: Liquid
pH Range: 4.7 – 6.0
Osmolality: 440 – 500 mOsm/kg
Concentrated: 100 X
Product Size: 100 mL
Classification: Animal Origin-Free
Green Features: Less waste, Sustainable packaging
Shipping Condition: Room Temperature

 

 

描述

Gibco® GlutaMAX™ supplement provides an alternative to L-Glutamine with improved stability and cell health. GlutaMAX™ supplement is suitable in both adherent and suspension culture of mammalian cells with no adaptation required. GlutaMAX™ supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ supplement is also available pre-supplemented in a variety of media formulations. Compared to L-Glutamine, GlutaMAX™ supplement:

  • Minimizes toxic ammonia build-up
  • Improves cell viability and growth
  • Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-Glutamine, GlutaMAX™ supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-Glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-Glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ supplement that is not possible with L-Glutamine.1

Remains stable across a wide range of temperatures
GlutaMAX™ supplement is room-temperature stable and ready to use. GlutaMAX™ supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -20°C for convenient storage and handling. GlutaMAX™ supplement is even stable at 37°C for a week or more.1

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
GlutaMAX™ supplement is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® GlutaMAX™ supplement made in our Scotland facility (35050-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
1Gibco® GlutaMAX™ Supplement Media brochure, 2003.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)