The NEBNext® dA-Tailing Module enables incorporation of a non-templated dAMP on the 3´ end of a blunt-ended DNA fragment. The module is optimized for use with the NEBNext dA-Tailing Module (NEB #E6053), and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 µg of input DNA.

 Klenow Fragment (3´→ 5´ exo–)

 NEBNext dA-Tailing Reaction Buffer

-20°C

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The NEBNext® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible

with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module (NEB #E6050) and the NEBNext dA-Tailing Module (NEB #E6053) and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 μg of input DNA.

 Quick T4 DNA Ligase

 NEBNext Quick Ligation Reaction Buffer

-20°C

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 –  RNA Sample Preparation
 –  Second Strand Synthesis of cDNA

The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow. 

The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module is Designed for Use with the

Following:

 –  NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771)

 –  NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)

 –  NEBNext Ultra II Ligation Module (NEB #E7595)

 –  NEBNext Ultra™ II Q5^® Master Mix (NEB #M0544)

 –  NEBNext^® Oligos for Illumina^® (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600,

    #E7535,  #E7350)

NEBNext^® Second Strand Synthesis Enzyme Mix

NEBNext^® Second Strand Synthesis Reaction Buffer