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TrypLE Express胰酶替代物，含EDTA，无酚红，100ml

发表于由

上海金畔生物科技代理销售TrypLE Express胰酶替代物，含EDTA，无酚红，100ml，欢迎访问官网了解更多产品信息。

TrypLE Express胰酶替代物，含EDTA，无酚红，100ml 
TRYPLE EXPRESS 
产品编号： 12604013  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 12604013

  

规格

Chelators: EDTA
Phenol Red Indicator: No Phenol Red
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Form: Liquid
pH Range: Check and Record
Osmolality: Check and Record
Concentrated: 1 X
Product Size: 100 mL
Reagent Type: Trypsin Replacement
Tests Performed: In Vitro Bioassay
Green Features: Energy efficient, Sustainable packaging, Less hazardous
Shipping Condition: Room Temperature

 

描述

 TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Life Technologies offers a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:

• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)

This TrypLE™ Express is modified as follows:

With Without
• EDTA • Phenol Red


The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Express’ exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

胰酶(0.05%), 0.5 g/L，HBSS-EDTA，含酚红，无钙、镁，500ml

发表于由

上海金畔生物科技代理销售胰酶(0.05%), 0.5 g/L，HBSS-EDTA，含酚红，无钙、镁，500ml，欢迎访问官网了解更多产品信息。

胰酶(0.05%), 0.5 g/L，HBSS-EDTA，含酚红，无钙、镁，500ml 
0.05% Trypsin-EDTA (1X), Phenol Red 
产品编号： 25300062  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 25300062

 

规格

Chelators: EDTA
Phenol Red Indicator: Phenol Red
Classification: Animal Origin
Form: Liquid
pH Range: 7.2 – 8.0
Osmolality: 270 – 310 mOsm/kg
Concentrated: 1 X
Product Size: 500 mL
Reagent Type: Trypsin
Tests Performed: In Vitro Bioassay
Green Features: Sustainable packaging
Shipping Condition: Wet Ice

 

描述

经过猪细小病毒和支原体实验测试。 HBSS (Hanks’ balanced salt solutions) 中含 0.5 g/L 胰蛋白酶 (1：250) 和 0.2 g/L EDTA•4Na，不含 CaCl2、MgCl2 • 6H2O 和 MgSO4 • 7H2O。含酚红。

胰酶(0.05%), 0.5 g/L，HBSS-EDTA，含酚红，无钙、镁，100ml

发表于由

上海金畔生物科技代理销售胰酶(0.05%), 0.5 g/L，HBSS-EDTA，含酚红，无钙、镁，100ml，欢迎访问官网了解更多产品信息。

胰酶(0.05%), 0.5 g/L，HBSS-EDTA，含酚红，无钙、镁，100ml 
Trypsin-EDTA (0.05%), phenol red 
产品编号： 25300054  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 25300054

 

规格

Chelators: EDTA
Phenol Red Indicator: Phenol Red
Classification: Animal Origin
Form: Liquid
pH Range: 7.2 – 8.0
Osmolality: 270 – 310 mOsm/kg
Concentrated: 1 X
Product Size: 100 mL
Reagent Type: Trypsin
Tests Performed: In Vitro Bioassay
Green Features: Sustainable packaging
Shipping Condition: Wet Ice

 

内容及储存

Storage conditions: -5°C to -20°C

Shelf life: 24 months from date of manufacture

 

描述

Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

• Quality testing
• Documented traceability
• Dual-site cGMP Manufacturing
 

This trypsin solution is modified as follows:

With
• EDTA
• Phenol Red

The complete formulation is available.
Quality testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.