标签归档：培养基

培养基DMEM/F-12，高糖，无酚红

发表于由

上海金畔生物科技代理销售培养基DMEM/F-12，高糖，无酚红，欢迎访问官网了解更多产品信息。

培养基DMEM/F-12，高糖，无酚红 
DMEM/F-12, HEPES, no phenol red 
产品编号： 11039021  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 11039021

 

规格

Glutamine: L-Glutamine
Phenol Red Indicator: No Phenol Red
Form: Liquid
HEPES Buffer: HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Serum Supplementation: Standard Serum Supplementation
Volume: 500 ml
Glucose: High Glucose
Product Size: 500 mL
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

内容及储存
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

培养基配方
11039 – DMEM/F-12, HEPES, no phenol red

描述

DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With Without
• L-glutamine • Phenol Red
• HEPES  


The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, Life Technologies manufactures DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

培养基F-12，无HEPES，500ml

发表于由

上海金畔生物科技代理销售培养基F-12，无HEPES，500ml，欢迎访问官网了解更多产品信息。

培养基F-12，无HEPES，500ml 
Ham’s F-12 Nutrient Mixture 
产品编号： 11765054  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 11765054

 

规格

Form: Liquid
Volume: 500 ml
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Product Size: 500 mL
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: Sodium Pyruvate
Green Features: Sustainable packaging
Shipping Condition: Room Temperature
 

 

描述

Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham’s F-12 is modified as follows:

With
• L-glutamine
• Phenol Red


The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.
Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

Neurobasal 基础培养基，出生前胎儿神经元干细胞培养

发表于由

上海金畔生物科技代理销售Neurobasal 基础培养基，出生前胎儿神经元干细胞培养，欢迎访问官网了解更多产品信息。

Neurobasal 基础培养基，出生前胎儿神经元干细胞培养 
Neurobasal® Medium (1X), Liquid 
产品编号： 21103049  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 21103049

 

规格

Cell Type: Stem Cells (Neural), Neuronal Cells (Pre-Natal)
Form: Liquid
Glutamine: No Glutamine
Amino Acids: No Aspartic Acid, No Glutamic Acid
Serum Level: Serum-Free
Culture Type: Feeder Cell-Free
Product Size: 500 mL
Classification: Serum-Free
Phenol Red Indicator: Phenol Red
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

 

描述

Neurobasal® 培养基是用于满足出生前和胎儿神经元细胞特殊需要的基础培养基，使用时需添加 GIBCO® B-27® 添加剂。 培养基用于海马神经元，大脑皮层和大脑其他区域的神经细胞的生长。Neurobasal® 培养基可以在不添加胶质细胞饲养层的情况下，可以长期和短期的维持神经细胞的同源群落存活。

添加您自己的添加剂
所有的Neurobasal® 培养基都不含L -谷氨酰胺，L -谷氨酸或天冬氨酸。Neurobasal® 培养基必须辅之以无血清添加剂(例如 B-27® 添加剂 或N-2 添加剂 ), 或血清和 0.5 mM L-谷氨酰胺 或 GlutaMAX™-I 添加剂 . 初次接种平板时，应加入25 µM 谷氨酸。

质量控制检测
所有的培养基都经过了PH，渗透压，内毒素的检测并且无细菌和真菌污染。
仅用于研究。不用于任何动物或人类的治疗或诊断使用。