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干粉培养基1640，无HEPES、丙酮酸钠、碳酸氢钠，10X1L

发表于由

上海金畔生物科技代理销售干粉培养基1640，无HEPES、丙酮酸钠、碳酸氢钠，10X1L，欢迎访问官网了解更多产品信息。

干粉培养基1640，无HEPES、丙酮酸钠、碳酸氢钠，10X1L 
RPMI 1640 Medium, Powder 
产品编号： 31800022  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 31800022

 

规格

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
HEPES Buffer: No HEPES
Serum Supplementation: Standard Serum Supplementation
Sodium Pyruvate Additive: No Sodium Pyruvate
Product Size: 10 x 1 L
Shipping Condition: Room Temperature

 

 

描述

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With Without
• L-glutamine • HEPES
• Phenol Red • Sodium Bicarbonate

The complete formulation is available.
Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g / L) and therefore requires a 5-10% CO2 environmentto maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

干粉培养基DMEM，高糖，无HEPES、丙酮酸钠、碳酸氢钠，10X1L

发表于由

上海金畔生物科技代理销售干粉培养基DMEM，高糖，无HEPES、丙酮酸钠、碳酸氢钠，10X1L，欢迎访问官网了解更多产品信息。

干粉培养基DMEM，高糖，无HEPES、丙酮酸钠、碳酸氢钠，10X1L 
DMEM, Powder, High Glucose 
产品编号： 12100046  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 12100046

 

规格

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: No Sodium Pyruvate
Product Size: 10 x 1 L
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Shipping Condition: Room Temperature

 

内容及储存

储存条件：2-8° C

运输条件：环境温度
保存期：自生产日期起36个月

 

描述

Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With Without
• High Glucose • Sodium Pyruvate
• L-glutamine • HEPES
• Phenol Red • Sodium Bicarbonate

The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

干粉培养基M199，无HEPES、丙酮酸钠，10X1L

发表于由

上海金畔生物科技代理销售干粉培养基M199，无HEPES、丙酮酸钠，10X1L，欢迎访问官网了解更多产品信息。

干粉培养基M199，无HEPES、丙酮酸钠，10X1L 
Medium 199, Powder 
产品编号： 31100035  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 31100035

 

规格

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: No Sodium Pyruvate
Glucose: Low Glucose
Product Size: 10 x 1 L
Shipping Condition: Room Temperature

 

描述

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This M199 is modified as follows:

With Without
• L-glutamine • HEPES
• Phenol Red • Sodium bicarbonate

The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle’s salts for use in a CO2 incubator, or with Hank’s salts for use without CO2.

In Vitro Diagnostic
Gibco® Medium 199 is manufactured in compliance with the requirements of ISO 13485: 2003 for the design, development, manufacture, and distribution of liquid and powder tissue culture media, sera, and reagents for cell culture research, industrial bioprocessing, and related markets.

Dual-Site cGMP Manufacturing
Gibco® Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001. For supply chain continuity, Life Technologies offers a comparable Gibco® Medium 199 product made in the Scotland facility (31100-027). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocol for details).

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)