标签归档：逆转录酶

逆转录酶SuperScript II，1万单位

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上海金畔生物科技代理销售逆转录酶SuperScript II，1万单位，欢迎访问官网了解更多产品信息。

逆转录酶SuperScript II，1万单位 
SuperScript® II Reverse Transcriptase 
产品编号： 18064014  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 18064014

 

规格

Optimal Reaction Temperature: 42° C
Final Product Size(s): 12.3 kb or less
Reverse Transcriptase: SuperScript® II
Ribonuclease H Activity: Reduced
Form: Frozen
Format: Tube(s)
Template: ssDNA, ssRNA, RNA-DNA Hybrid
Sensitivity: Medium
Product Size: 10,000 units
Concentration: 200 U⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Wet Ice
Number of Reactions: 50 Reactions

 

内容及储存

SuperScript® II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.

Store at -20°C. Guaranteed stable for 6 months when properly stored.

 

描述

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.

• Yield – Reduced RNase H activity results in greater first-strand cDNA yields
• Size of cDNA – Generation of RT-PCR products up to 12 kb
• Thermostability – Full activity at 42°C
• Flexibility – Uses either total or poly(A)+ RNA

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

SuperScript® III Reverse Transcriptase 逆转录酶SuperScript III，1万单位，反转录酶

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上海金畔生物科技代理销售SuperScript® III Reverse Transcriptase 逆转录酶SuperScript III，1万单位，反转录酶，欢迎访问官网了解更多产品信息。

SuperScript® III Reverse Transcriptase 逆转录酶SuperScript III，1万单位，反转录酶 
SuperScript® III Reverse Transcriptase 
产品编号： 18080044  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 18080044

 

规格

Optimal Reaction Temperature: 50° C
Final Product Size(s): 12.3 kb or less
Reverse Transcriptase: SuperScript® III
Ribonuclease H Activity: Reduced
Form: Frozen
Format: Tube(s)
Quantity: 10,000 units
Template: ssRNA
Sensitivity: Medium
Product Size: 10,000 units
Concentration: 200 U⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice
Number of Reactions: 50 Reactions

 

内容及储存

SuperScript® III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.

Store at -20°C. Guaranteed stable for 6 months when properly stored.

 

描述

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR ofa specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

• Thermostability – Half life of 220 minutes at 50°C
• Yield – Reduced RNase H activity for more full-length cDNA
• Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
• Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE

Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as a template•primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

 

逆转录酶M-MLV，4万单位

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上海金畔生物科技代理销售逆转录酶M-MLV，4万单位，欢迎访问官网了解更多产品信息。

逆转录酶M-MLV，4万单位 
M-MLV Reverse Transcriptase 
产品编号： 28025013  CAS号：  
分子式：   分子量：  
EINECS编号：  

货号： 28025013

 

规格

 

Optimal Reaction Temperature: 37° C
Final Product Size(s): 7 kb or less
Reverse Transcriptase: M-MLV Reverse Transcriptase
Ribonuclease H Activity: Reduced
Format: Tube(s)
Template: ssDNA, ssRNA, RNA-DNA Hybrid
Sensitivity: Low
Product Size: 40,000 units
Concentration: 200 U⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Wet Ice
Number of Reactions: 200 Reactions

 

内容及储存

 

M-MLV RT is supplied with a vial of 5X first strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2 ], and a vial of 100 mM DTT. Store at -20°C. Guaranteed stable for 6 months when properly stored.

 

 

描述

 

M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.

• Thermostability – Optimal activity at 37°C
• Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)

Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.

Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (6).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C (6).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.