上海金畔生物科技代理销售SuperScript® III Reverse Transcriptase 逆转录酶SuperScript III,1万单位,反转录酶,欢迎访问官网了解更多产品信息。
SuperScript® III Reverse Transcriptase 逆转录酶SuperScript III,1万单位,反转录酶 |
SuperScript® III Reverse Transcriptase |
产品编号: |
18080044 |
CAS号: |
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分子式: |
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分子量: |
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EINECS编号: |
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货号: 18080044
规格
Optimal Reaction Temperature: |
50° C |
Final Product Size(s): |
12.3 kb or less |
Reverse Transcriptase: |
SuperScript® III |
Ribonuclease H Activity: |
Reduced |
Form: |
Frozen |
Format: |
Tube(s) |
Quantity: |
10,000 units |
Template: |
ssRNA |
Sensitivity: |
Medium |
Product Size: |
10,000 units |
Concentration: |
200 U⁄µl |
Enzyme Function: |
RNA dependent DNA Polymerase |
Shipping Condition: |
Dry Ice |
Number of Reactions: |
50 Reactions |
内容及储存
SuperScript® III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.
描述
SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR ofa specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.
• Thermostability – Half life of 220 minutes at 50°C
• Yield – Reduced RNase H activity for more full-length cDNA
• Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
• Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3´ and 5´ RACE
Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as a template•primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.