Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州，是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材，同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线，不仅为全世界晶体研究人员的工作带来了便捷，而且也受益于科研人员的回馈不断发展自己，目前已成为晶体研究领域最受信赖的品牌之一。
上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商，我们将竭诚为您服务，欢迎新老客户咨询。

Hampton research主要产品：

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商，该公司的产品目录是由上海金畔生物科技有限公司提供，该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类：Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test（PCT预结晶试验）
应用：结晶筛选，确定相应的样品浓度，PCT试验用来预结晶结晶筛选，以确定适当的蛋白质浓度

2. Slice pH（PH切片）

应用：溶解度和结晶筛选

3. Solubility & Stability Screen（溶解度和稳定性的屏幕）

应用：溶解性和稳定性屏幕的设计，以协助识别解决方案的条件，促进蛋白质的溶解性和稳定性，并尽量减少蛋白质沉淀。

4. Index

应用：不同的试剂系统的结晶屏幕的蛋白质复合物，多肽，核酸，水溶性小分子。

5. Crystal Screen（晶体屏幕）

应用：
主屏幕的可溶性肽，蛋白质，核酸，和水溶性小分子。稀疏矩阵的加法屏幕
5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶
6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶
7.Crystal Screen? & Crystal Screen? HT 用于蛋白质，复合物，缩氨酸，核酸以及水溶性小分子的传统的经典结晶试剂
8.Crystal Screen 2?用于蛋白质，缩氨酸，核酸以及水溶性小分子的结晶
9.Crystal Screen Lite?用于蛋白质，缩氨酸和核酸的结晶试剂
10.Crystal Screen Cryo?用于蛋白质，缩氨酸，核酸的结晶
11.Grid ScreenTM用于蛋白质的结晶，分为七小类。
12.Quik Screen?用于可溶性蛋白的结晶
13.Quik Optimize?快速优化试剂，与Quik Screen? 或 custom screen配套使用
14.Low Ionic Strength Screen?用于完整的单克隆抗体，单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶
15.Nucleic Acid Mini Screen?用于核酸片段的结晶化
16.Natrix?用于核酸，蛋白核酸聚合物的结晶
17.MembFac?& MembFac? HT用于膜蛋白的结晶
18.Additive Screen?增强或改变样品可溶性的试剂
19.Detergent Screen?去垢剂
20.Heavy Atom Screens重原子结晶试剂
21.Izit Crystal Dye?蛋白染色试剂，用于区分蛋白晶体和盐晶体

第二类：Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

1. Solubility & Stability Individual Reagents
2. Slice pH Individual Reagents
3. Ionic Liquid Screen Individual Reagents
4. Proti-Ace & Proti-Ace 2 Individual Reagents
5. PCT Individual Reagents
6. Silver Bullets & Silver Bullets HT Individual Reagents
7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
8. Index • Index HT Individual Reagents
9. PEGRx 1 Individual Reagents
10. PEGRx 2 Individual Reagents
11. SaltRx Individual Reagents
12. PEG/Ion Individual Reagents
13. Crystal Screen Lite Individual Reagents
14. Crystal Screen Cryo Individual Reagents
15. Crystal Screen 2 Cryo Individual Reagents
16. Grid Screen Ammonium Sulfate Individual Reagents
17. Grid Screen PEG 6000 Individual Reagent
18. Grid Screen Sodium Chloride Individual Reagents
19. Grid Screen PEG/LiCl Individual Reagents
20. Quik Screen Individual Reagents
21. Natrix Individual Reagents

第三类：Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体
2.Optimize-Organics (Volatile) 挥发性有机物
3.Optimize-Organics (Non-Volatile)非挥发性的有机物
4.Optimize-Salts 盐类
5.Optimize-Buffers缓冲液
6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB
7.Optimize-Reducing Agent还原剂-TCEP氢氯化物
8.Optimize-Cryoprotectants
9.Optimize-Oils用于结晶的油类
10.Optimize-Silica Hydrogel硅胶
11.Optimize-LM Agarose低熔点琼脂糖

第四类：StockOptions Kits

1. StockOptions Salt
2. StockOptions pH
3. StockOptions Buffer
4. CryoPro

第五类：Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
2.48 Well Crystallization Plates 48孔晶体板
3.72 Well Crystallization Plates72孔晶体板
4.96 Well Crystallization Plates 96孔晶体板
5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶
6.Cover Slides & Related Tools
7.Glass Sitting Drop Rods用于坐滴的结晶
8.Micro-Bridges?微桥-坐滴结晶的支撑物
9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板
10.Sealants, Sealing Grease, Oils, Applicator密封油脂，油及其它器具
11.Sealing Films, Tapes, Mats & Covers
12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化
13.Granada Crystallization Box

第六类：Tools, Seeding & Resin（结晶工具、树脂）

1. Tungsten-carbide Glass Cutter
2. X-Acto Gripster Knife
3. Micro-Tools™ Set
4. Micro-Tools II™ Set
5. Forceps
6. Crystal Probe™
7. Crystal Pencil
8. Seed Bead
9. Seeding Tool

第七类：Cryocrystallography

1. CrystalCap HT™ Systems
2. CrystalCap Magnetic™ Systems
3. CrystalCap™ Systems
4. CryoLoops & MicroTubes
5. Cryo Tools
6. Canes, Sleeves, Coders & Boxes
7. Dewars

第八类：Capillary Mounts & Supplies（毛细管座椅支架供应）
第九类：Goniometer Heads & Supplies（测角头及用品）
第十类：Xenon Derivatization（氙气衍生物）
第十一类：Labels & Pens（标签和记录笔）
第十二类：Protein Crystallization Standards（蛋白质结晶的标准）
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Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理（活性荧光染料）   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理（蛋白结晶） whatman代理（滤膜滤纸） GE代理（蛋白纯化） Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理（糖蛋白分析产品）  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton PEGRx
PEGRx 1 • PEGRx 2 • PEGRx HT

Applications   主要是调节样本的PH值
  Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。PEGRx 1 contains 48 unique reagents, 10 ml each.
PEGRx 2 contains 48 unique reagents, 10 ml each
PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

PEGRx

DesR crystals grown using Hampton Research PEGRx, Jin Ho Moon, Korea University.

Hampton PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications
  Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.
PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.¹ Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.
PEG/Ion contains 48 unique reagents, 10 ml each.
PEG/Ion 2 contains 48 unique reagents, 10 ml each.
PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystals of Peptidoglycan Recognition Protein (PGRP) from camel milk grown using the Hampton Research PEG/Ion screen. Sharma, P., Singh, N., Sinha, M., Sharma, S., Perbandt, M., Betzel, C., Kaur, P., Srinivasan, A. & Singh, T.P. All India Institute of Medical Sciences, Department of Biophysics, New Delhi, India. CAT NO NAME DESCRIPTION HR2-126 PEG/Ion Screen 10 ml, tube format HR2-098 PEG/Ion 2 Screen 10 ml, tube format HR2-139 PEG/Ion HT 1 ml, Deep Well block format